This microscope is excellent for simultaneous multi-color, deep, in situ imaging of excised or live samples, including tissues and whole animals under anesthesia. CellSens Dimension with deconvolution (Olympus) and Imaris for Cell Biologists 9.2.1 (Bitplane) software are available on a separate HP Z8 workstation for data analysis. This microscope is housed in an independent, secured room with significant light mitigation, inside a custom-made dark box. Laser output power can also be software adjusted and tuned through the imaging depth.Ī sample warming pad (Stoelting) and isoflurane anesthesia is available for imaging small animals. Other features include auto-alignment of the lasers, beam- shaping for improved deep focus, and a motorized correction collar for the 25X objective to correct for refractive index over depth to provide maximum brightness, contrast and resolution through the entire imaged volume. This system is controlled with advanced Olympus Fluoview acquisition software, a motorized nosepiece and X-Y Prior Z-Deck stage allowing for multipoint, tiling, Z-series time-lapse imaging, etc. A 20x air, NA 0.45 WD 8.2 mm is available for GRIN lens work. Available multi-photon optimized objectives include a 5x NA 0.10 air WD 20 mm, 10x NA 0.60 water immersion WD 3.3 mm, 25x water immersion NA 1.05 WD 2.0 mm and 60x water immersion NA 1.10 WD 1.5 mm. The hybrid scanning system with galvanometer and resonance scanners is ideal for high-temporal resolution imaging.
All optics are designed for long wavelength transmittance and reflectance. The microscope has four PMT detectors, two enhanced and two high-sensitivity GaAsP. The microscope is equipped with two separate, pre-chirped, tunable, pulsed IR lasers, a Spectra Physics Insight X3 (680-1300 nm) and Spectra Physics MaiTai eHP DeepSee (690-1040 nm). This is a dedicated multi-photon microscope built on an Olympus BX63LF upright frame. ‘Gemini’ – Olympus FVMPE-RS multi-photon microscope. You might also like: The Aunty No 1 Hindi Dubbed Torrent Download.
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